Understanding the Mode of Action of Your Small Bioactive Molecule

Charnwood Molecular and Hybrigenics Services have launched a new technology that identifies interactions between small molecules and protein targets. The new ULTImate YChemH service is the result of a two-year long research and development phase, which was supported by European funding under the Eurostars programme.

ULTImate YChemH is a chemical biology tool for direct targets identification. This method is based on the well-established yeast two-hybrid (Y2H) technology.


  • Identify hit targets and decipher mechanisms of action following a phenotypic screening (drug profiling, target deconvolution)
  • Study the off-target effects of an active compound
  • Support drug repositioning in new therapeutic areas
  • Evaluate safety of chemicals (toxico-proteomics)


The small molecule of interest is used as a bait to screen highly complex protein domain libraries.The small molecule – protein interactions are detected thanks to the reconstitution of an active transcription factor from DNA Binding Domain (DBD) and Activation Domain (AD) moieties. When an interaction takes place, the bait derivative bridges the gap between the DBD and the AD. 


This enables the expression of the reporter gene and subsequent yeast growth on a selective medium. Positive clones are then analyzed by sequencing to identify the protein partners. 

Charnwood uses its expertise to link bioactive molecules for further analysis by Paris, France-based Hybrigenics sevices.

Charnwood Molecular has developed libraries of linker building blocks, which are ready to attach to the client’s molecules. 




For further information about the service and cases studies please contact Sylvain Blanc @ s.blanc@charnwood-molecular.com or visit Hybrigenics Services website:



For further information on the ULTImate YChemH technology, please visit this link:

ULTImate YChemH